Specific fibrinogen quantitation by electroimmunodiffusion in agarose gel containing heparin.

Abstract

In the determination of plasma fibrinogen by electroimmunodiffusion, commercial sodium heparin, previously included in the agarose gel, by interacting with fibrinogen molecules, enhances their anodic mobility more strongly than after carbamylation with potassium cyanate. The proposed method provides an increase in sensitivity and specificity which practically eliminates the influence of fibrinogen degradation products.

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